rat lncrna/mrna microarray  (Arraystar inc)

Journal: Journal of Pain Research

Article Title: Long Non-Coding RNA and mRNA Profiles in the Spinal Cord of Rats with Resiniferatoxin-Induced Neuropathic Pain

doi: 10.2147/JPR.S368599

Figure Lengend Snippet: The expression profile analysis of the lncRNAs/mRNAs and overview of microarray analysis. The purity and quantity of RNA were evaluated by a K5500 micro-spectrophotometer. RNA purity was acceptable if A260/A230 ≥ 0.5 and A260/A280 ≥ 1.5. RNA integrity was acceptable when RIN value ≥ 7 detected by Agilent 2200 RNA assay. Gel electrophoresis ( A ) was conducted to assess the genomic DNA contamination. Expression signal distribution after every mRNA array dataset was normalized ( B ). The microarray heat map of spinal dorsal horn tissues in the PHN model and analysis of differentially expressed lncRNAs (Vehicle group: A1, A2 and A3; PHN model group: B1, B2 and B3) ( C and D ). Red color represented that the relative expression was high and green color represented that the relative expression was low. For advanced data analysis, all biological replicates were pooled and calculated to identify differentially expressed mRNAs based on the threshold of |Fold change| ≥ 1.5 and P ≤ 0.05. Unsupervised hierarchical clustering analysis was conducted to demonstrate the correlation of expression profiles between treatment and biological replicates.

Article Snippet: The RiboArray microArray covering 29,659 unique rat mRNAs and 6713 unique rat lncRNAs (refseq60) was provided by Ribobio (Ribobio Co. Guangzhou, China) via the Combimatrix platform (Washington-Seattle, USA).

Techniques: Expressing, Microarray, Spectrophotometry, Nucleic Acid Electrophoresis

Journal: Journal of Pain Research

Article Title: Long Non-Coding RNA and mRNA Profiles in the Spinal Cord of Rats with Resiniferatoxin-Induced Neuropathic Pain

doi: 10.2147/JPR.S368599

Figure Lengend Snippet: LncRNA-mRNA interaction network diagram. The red box represents lncRNAs, the blue circle represents predicted mRNAs, and the line represents the relationship between lncRNAs and mRNAs. All LncRNA target genes were obtained by LncRNA target gene prediction. The interaction between lncRNAs and target genes was obtained. LncRNA-mRNA interaction network diagram was drawn. Due to the large number of results of this project, lncRNAs with more than 20 target genes were screened ( A ). A network diagram of the relationships between overlapping genes. The intersection of mRNAs predicted by lncRNAs and differential mRNA results was revealed, and the interaction network diagram between the intersection genes and the corresponding lncRNA was drawn ( B ). An overview of the aberrant lncRNAs by analyzing the microarray data ( C ).

Article Snippet: The RiboArray microArray covering 29,659 unique rat mRNAs and 6713 unique rat lncRNAs (refseq60) was provided by Ribobio (Ribobio Co. Guangzhou, China) via the Combimatrix platform (Washington-Seattle, USA).

Techniques: Microarray

Journal: Frontiers in Molecular Biosciences

Article Title: Lipopolysaccharide Alters the m6A Epitranscriptomic Tagging of RNAs in Cardiac Tissue

doi: 10.3389/fmolb.2021.670160

Figure Lengend Snippet: M 6 A single-base site qPCR was used to confirm the microarray data for the top four methylated lncRNAs and top six methylated mRNAs in left ventricle tissue between the LPS and control groups. * p < 0.05 versus the Ctrl group. Ctrl, control; LPS, lipopolysaccharide.

Article Snippet: The cRNAs were combined and hybridized onto an Arraystar Rat mRNA&lncRNA Epitranscriptomic Microarray (4 × 44K, Arraystar) that contained 27,770 mRNA and 10,582 lncRNA degenerate probes.

Techniques: Microarray, Methylation, Control

Journal: Journal of Neuroinflammation

Article Title: The potential roles of m 6 A modification in regulating the inflammatory response in microglia

doi: 10.1186/s12974-021-02205-z

Figure Lengend Snippet: mRNA and lncRNA m 6 A modification profile changes in different primary rat microglia phenotypes. Hierarchical clustering of all samples revealed the non-random partitioning of samples into three major groups: M1-L vs M0-L; M2-L vs M0-L; and M2-L vs M1-L. Each column represents one sample and each row represents one mRNA ( A ) or lncRNA probe set ( B )

Article Snippet: The Cy3- and Cy5-labeled cRNA were mixed and hybridized to a rat m 6 A mRNA and lncRNA Epitranscriptomic Microarray (4 × 44 K, Arraystar) that contained 27,770 mRNA and 10,582 lncRNA.

Techniques: Modification

Journal: Journal of Neuroinflammation

Article Title: The potential roles of m 6 A modification in regulating the inflammatory response in microglia

doi: 10.1186/s12974-021-02205-z

Figure Lengend Snippet: Schematic overviews of the signaling pathways in which 10 m 6 A lncRNAs are probably involved. Ten m 6 A methylated lncRNAs, their regulated mRNAs and proteins are in the 14 upregulated signaling pathways. The red circles represent proteins, the green arrows represent the regulatory relationship of lncRNA to mRNA, the orange arrows show the mRNA and protein pair, and blue arrows indicate signaling pathways that proteins are involved

Article Snippet: The Cy3- and Cy5-labeled cRNA were mixed and hybridized to a rat m 6 A mRNA and lncRNA Epitranscriptomic Microarray (4 × 44 K, Arraystar) that contained 27,770 mRNA and 10,582 lncRNA.

Techniques: Protein-Protein interactions, Methylation